Microbe Mission B/C

[Nano1llus10n]

Lol ok.

Pick one option in each. When changing objectives from scanning to low to high powers,

a. the size of the field of view increases/decreases.
b. the field of view gets lighter/darker.
c. the size of the image increases/decreases.
d. the resolution increases/decreases.
e. the working distance increases/decreases.
f. the depth of focus increases/decreases.

[whythelongface]

Lol ok.

Pick one option in each. When changing objectives from scanning to low to high powers,

a. the size of the field of view increases/decreases.
b. the field of view gets lighter/darker.
c. the size of the image increases/decreases.
d. the resolution increases/decreases.
e. the working distance increases/decreases.
f. the depth of focus increases/decreases.

Answer (Click to Show Hidden Text)

a. decreases
b. darker
c. increases
d. increases
e. decreases?
f. decreases?

Can you explain the last two?

[Nano1llus10n]

Lol ok.

Pick one option in each. When changing objectives from scanning to low to high powers,

a. the size of the field of view increases/decreases.
b. the field of view gets lighter/darker.
c. the size of the image increases/decreases.
d. the resolution increases/decreases.
e. the working distance increases/decreases.
f. the depth of focus increases/decreases.

Answer (Click to Show Hidden Text)

a. decreases
b. darker
c. increases
d. increases
e. decreases?
f. decreases?

Can you explain the last two?

Nice! You got them all correct!

Explanation (Click to Show Hidden Text)

E. working distance is defined as the distance between the front lens of the objective and the closest surface of the coverslip when the specimen is in sharp focus. Therefore, when you move objectives, the distance gets smaller since the objective lens is bigger. F. when you shift from a lower objective to a higher objective, the image becomes blurry. This is a decrease in the depth of focus.

Your turn!

[whythelongface]

Question (Click to Show Hidden Text)

What is an NTD? Name one disease on the Microbe List that causes an NTD.

[Alex-RCHS]

Lol ok.

Pick one option in each. When changing objectives from scanning to low to high powers,

a. the size of the field of view increases/decreases.
b. the field of view gets lighter/darker.
c. the size of the image increases/decreases.
d. the resolution increases/decreases.
e. the working distance increases/decreases.
f. the depth of focus increases/decreases.

I feel like d should actually be decreases. It depends on how you define resolution. I’ve always thought of it as the minimum distance between two points required to distinguish between them. In this case a higher magnification objective with “better” resolution would have lower resolution, because two points could be closer together and still be viewed as distinct. However, it’s possible that I’m misunderstanding it, or you had a different measurement in mind.

Edit: nevermind, I’m pretty sure I’m wrong. What i was referring to was called point (or lateral) resolution, and i think it’s not usually used in microscopy. Furthermore, even when it is used “higher resolution” means (somewhat paradoxically) that a smaller distance can be accommodated between two distinguishable points.

My bad.

Also, I’ll take a stab at the question:

guess (ish) (Click to Show Hidden Text)

NTD stands for NeuroToxic Disease...? I’m not sure about the T, especially. 

I’ll guess schistosmiasis. Is this what can be caused by the larvae of schistosomes during the parenteral phase of infection if they crawl into the CNS?

[whythelongface]

Also, I’ll take a stab at the question:

guess (ish) (Click to Show Hidden Text)

NTD stands for NeuroToxic Disease...? I’m not sure about the T, especially. 

I’ll guess schistosmiasis. Is this what can be caused by the larvae of schistosomes during the parenteral phase of infection if they crawl into the CNS?

Have another stab.

Also, higher resolution just means higher resolving power. This means that the ability to discriminate between two points close together is increased, nothing more.

[sciduck]

Also, I’ll take a stab at the question:

guess (ish) (Click to Show Hidden Text)

NTD stands for NeuroToxic Disease...? I’m not sure about the T, especially. 

I’ll guess schistosmiasis. Is this what can be caused by the larvae of schistosomes during the parenteral phase of infection if they crawl into the CNS?

Have another stab.

I think the T is (Click to Show Hidden Text)

tropical. So Dengue, maybe? I don't know what N stands for though, and I don't even know what to guess.

[Nano1llus10n]

Also, I’ll take a stab at the question:

guess (ish) (Click to Show Hidden Text)

NTD stands for NeuroToxic Disease...? I’m not sure about the T, especially. 

I’ll guess schistosmiasis. Is this what can be caused by the larvae of schistosomes during the parenteral phase of infection if they crawl into the CNS?

Have another stab.

I think the T is (Click to Show Hidden Text)

tropical. So Dengue, maybe? I don't know what N stands for though, and I don't even know what to guess.

I've heard of this before... (Click to Show Hidden Text)

I forgot the exact acronym but I'm pretty sure that sciduck is correct about the tropical part. One example I remember is a hookworm infection

[whythelongface]

NTD is (Click to Show Hidden Text)

Neglected tropical disease. Hookworms are one example of a pathogen that causes NTDs.

It's sciduck's turn.

[sciduck]

How do quinolones work?
A. Inhibit cell wall synthesis
B. Inhibit protein synthesis
C. Inhibit nucleic acid replication/transcription
D. Injure the plasma membrane
E. Inhibit essential metabolite synthesis